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KMID : 0366220140490010049
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Korean Journal of Hematology
2014 Volume.49 No. 1 p.49 ~ p.53
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The caspase-3 inhibitor (peptide Z-DEVD-FMK) affects the survival and function of platelets in platelet concentrate during storage
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Reza Shiri
Fatemeh Yari
Minoo Ahmadinejad
Shahram Vaeli
Mohammad Reza Tabatabaei
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Abstract
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Background: Although apoptosis occurs in nucleated cells, studies show that this event also occurs in some anucleated cells such as platelets. During storage of platelets, the viability of plate-lets decreased, storage lesions were observed, and cells underwent apoptosis. We inves-tigated the effects of caspase-3 inhibitor on the survival and function of platelets after different periods of storage.
Methods: Platelet concentrates were obtained from the Iranian Blood Transfusion Organization in plastic blood bags. Caspase-3 inhibitor (Z-DEVD-FMK) was added to the bags. These bags along with control bags to which no inhibitor was added were stored in a shaking incubator at 22oC for 7 days. The effects of Z-DEVD-FMK on the functionality of platelets were ana-lyzed by assessing their ability to bind to von Willebrand factor (vWF) and to aggregate in the presence of arachidonic acid and ristocetin. Cell survival was surveyed by MTT assay.
Results: At day 4 of storage, ristocetin-induced platelet aggregation was significantly higher in the inhibitor-treated (test) than in control samples; the difference was not significant at day 7. There was no significant difference in arachidonic acid-induced platelet aggregation between test and control samples. However, at day 7 of storage, the binding of platelets to vWF was significantly higher in test than in control samples. The MTT assay revealed significantly higher viability in test than in control samples at both days of study.
Conclusion: Treatment of platelets with caspase-3 inhibitor could increase their functionality and survival.
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KEYWORD
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Platelet concentrate, Storage, Caspase inhibitor, von Willebrand factor
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